Fig. 2

Effect of miR-212-3p expression on the neoplastic potential of group 3 MB cancer cells. A MiR-212-3p expression restoration via transient (HDMB03 and D425) and Dox inducible (HDMB03) systems by RT-PCR. B Cell proliferation assays (MTT and WST-1) showing significantly reduced proliferative potential with both transient and stable expression of miR-212-3p in a time- and dose-dependent manner in group 3 MB cells. Similar growth-restrictive properties demonstrable via colony formation and transwell migration (C and D), and wound healing assays (E) with both transient and stable expression of miR-212-3p in HDMB03 cells. Data presented as mean ± SD from experiments done in triplicate and analyzed using Student’s t-test; *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar: 100 μm