Table 2 Primers and conditions used in PCR and sequencing
Primer or Step | Sequence or condition |
|---|---|
PCR Forward | 5’-AGCCCTTCTCCCCGCCTCCCACTGT-3’ |
PCR Reverse | 5’-CTCCGCCACCTGCTCCTTCACCTCG-3’ |
Sequencing primer | GATGGACGAGACCATG |
Initial denaturation | 98 ˚C for 4 min |
Denaturation (× 35 cycles) | 98 ˚C for 10 s |
Annealing (× 35) | 68 ˚C for 30 s |
Extension (× 35) | 72 ˚C for 45 s |
Final extension | 72 ˚C for 10 min |