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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Assembly of α-synuclein and neurodegeneration in the central nervous system of heterozygous M83 mice following the peripheral administration of α-synuclein seeds

Fig. 3

Synucleinopathy in M83± mice following intravenous injection of assembled A53T α-synuclein. A Staining of midbrain periaqueductal grey (PAG) and lumbar spinal cord (ventral horn) for pS129 α-synuclein (Wako pSyn#64). Arrows denote perikaryal and arrowheads neuritic staining. Scale bar, 50 μm. B Immunoblots of sarkosyl-insoluble material from brain and spinal cord using an antibody specific for pS129 α-synuclein (Abcam ab51253). C Negative-stain immunoelectron microscopy of α-synuclein filaments from brain and spinal cord using an antibody specific for pS129 α-synuclein (Abcam ab51253). Scale bar, 50 nm. D Kaplan–Meier survival curves following intravenous injection of different amounts of assembled A53T α-synuclein or PBS. Gold, 1 mg; green, 100 μg; blue, 10 μg; magenta, 1 μg; purple, PBS

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