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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Assembly of α-synuclein and neurodegeneration in the central nervous system of heterozygous M83 mice following the peripheral administration of α-synuclein seeds

Fig. 1

Synucleinopathy in M83± mice following oral (AE) and nasal (FI) administration of assembled A53T α-synuclein. A Staining of midbrain periaqueductal grey (PAG) and lumbar spinal cord (ventral horn) for pS129 α-synuclein (Wako pSyn#64). Arrows denote perikaryal and arrowheads neuritic staining. Scale bars, 50 μm. B Immunoblots of sarkosyl-insoluble material from brain and spinal cord using an antibody specific for pS129 α-synuclein (Abcam ab51253). C Negative-stain immunoelectron microscopy of filaments from brain and spinal cord using an antibody specific for pS129 α-synuclein (Abcam ab51253). Scale bars, 50 nm. D Kaplan–Meier survival curves following oral administration of assembled A53T α-synuclein (red) or PBS (purple). E Motor impairment on rotarod testing following oral administration of assembled A53T α-synuclein (red) compared to controls (purple). Unpaired one-tailed t-test for 6-month time-point. *p = 0.03. F Staining of midbrain periaqueductal grey (PAG) and lumbar spinal cord (ventral horn) for pS129 α-synuclein (Wako pSyn#64). Arrows denote perikaryal and arrowheads neuritic staining. Scale bars, 50 μm. G Immunoblots of sarkosyl-insoluble material from brain and spinal cord using an antibody specific for pS129 α-synuclein (Abcam ab51253). H Negative-stain immunoelectron microscopy of filaments from brain and spinal cord using an antibody specific for pS129 α-synuclein (Abcam ab51253). Scale bars, 50 nm. I Kaplan–Meier survival curves following nasal administration of assembled A53T α-synuclein (orange) or PBS (purple)

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