Fig. 6

Altered protein expression in the cortex and hippocampus of GL mice. a, b LIN28A immunostaining of GL and CTRL brains used for proteome analyses at P0 (a) and P15 (b). c Significant altered proteins (with fraction) and overall detected proteins for P0 cortex (Ctx), P15 Ctx and P15 hippocampus (Hc). d Hierarchical clustering of significantly differentially abundant proteins in P0 GL cortices when compared to CTRL. e Specific gene ontology (GO) terms showed the top terms “regulation of synapse structure or activity” and “synapse maturation” for unspecific proteins and “RNA binding” and “mRNA processing” for overexpressed proteins. f Hierarchical clustering of significantly differentially abundant proteins in P15 GL cortices when compared to CTRL. g Specific GO terms showed the top terms “regulation of mRNA splicing, via spliceosome” and “ribose phosphate biosynthetic process” for decreased protein abundances and “iron ion transport” for increased protein abundances. h Hierarchical clustering of significantly differentially abundant in P15 GL hippocampi when compared to CTRL. i Specific GO terms showed the top terms “coated vesicle membrane” and “negative regulation of peptidyl-serine phosphorylation” for decreased protein abundances and “response to calcium ion” and “negative regulation of neuron projection regeneration “ for increased protein abundances. Scale bar in d is for d, f and h. Scale bar of node size significance is for e, g and I; scale bar of cluster color (shows the proportion of genes from each cluster that are associated to the term) in e (for e, g and i) is red for increased terms (100%-60%); scale bar of cluster color is blue for decreased terms (100%-60%). A term was called specific with > 60% genes being part of respective cluster, scale bar of cluster color is grey for unspecific terms (< 60%). Scale bar in a and b is 50 µm. CTRL n = 5, GL n = 5 for d, CTRL n = 3, GL n = 3 for f and h. P values as shown in GO term description