Fig. 3

GL mice show LIN28A overexpression in neurons of the cortex and hippocampus. a Costainings of LIN28A (brown) and SOX2 (red) show LIN28A positivity in the SOX2-positive cortical ventrical zone. b Costainings of LIN28A (brown) and OLIG2 (red) show lack of LIN28A expression in the OLIG2-positive ventrical zone of the lateral and medial ganglionic eminences. Costaining is found in the region of the developing hippocampus (*). (c) NeuN-positive neurons in the cortex express LIN28A (c, d, arrowhead). No colocalization of LIN28A-positive cells and OLIG2-positive cells in the cerebral cortex at P15 (e, f). g NeuN-positive neurons in the pyramidal layer of the hippocampus strongly express LIN28A (arrowhead). No colocalization of LIN28A-positive cells and OLIG2-positive in the hippocampus (h). For investigation of subcellular expression patterns, high magnifications of LIN28A stained sections of GL brains are shown at different time points. At embryonal and postnatal stages, LIN28A was found in the cytoplasm (i). Scale bar in a is 500 μm for a and b; scale bar inset a is 50 μm for a and b; scale bar panel c is 500 μm for c and e; scale bar panel d is 50 μm for d and f; scale bar panel g is 100 μm for g and h; scale bar in i is 20 μm for all time points; Ctx = cortex, Hc = hippocampus