Fig. 6

Lysosomal disruption with Chloroquine leads to increased pathology but not a change in distribution. a Neurons treated in 96-well plates with 25 ng per well of either AD1-P1 or PFF for 6 h followed by a 30 min pulse of either PBS or 25 uM Chloroquine (ChQ) and fixed after 10 days. Scale bar 50 µm. b Quantitation of total 81A signal for neurons treated as in a. c Same quantitation of total 81A as in b, with totals for Somatic and Neuritic pathology shown. d Percent of total 81A pathology in somatic inclusions from neurons treated as in a. Totals are the average of 3 replicate wells. Error bars represent the standard deviation. Significance compared to PFF treated neurons was determined by student t-test (*p < 0.05, **p < 0.01)