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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Alpha-synuclein from patient Lewy bodies exhibits distinct pathological activity that can be propagated in vitro

Fig. 1

Purification and characterization of Frontal Cortex Lewy Bodies (LB) from AD and PDD brains. a Immunohistochemistry images of α-Syn positive LBs (arrows) and LNs (arrowheads) in frontal cortex of postmortem brain samples of 3 of the AD and 3 of the PDD patients used for this study before sequential extraction and purification. Scale bar 50 µm. b Schematic representation of the sequential extraction procedure used to purify pathological α-Syn from frontal cortex of postmortem human brain tissue. c Western blot analysis of purified Sarkosyl-insoluble α-Syn in AD and PDD brains probed with total α-Syn (HuA) and Pα-Syn (81A) antibodies. d ELISA and BCA analysis of purified pathological α-Syn in AD and PDD brains

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