Fig. 5

LRRK2 kinase inhibition increases colocalization of α-synuclein and presynaptic markers in the striatum A Confocal microscope images of α-synuclein (green) and vGLUT1 (red) in the dorsal striatum in mice fed with control (top panels) or 175 mg/kg PF-360 (lower panels) for 7 days. The images on the right represent all colocalized pixels (black) between α-synuclein and vGLUT1 immunostainings. Scale bar = 10 µm. B Confocal microscope images of α-synuclein (green) and dopaminergic terminal marker DAT (red) in the mouse dorsal striatum. The top panels are from control mice and the lower panels are from the mice fed PF-360 chow for 7 days. The images on the right represent all colocalized pixels (black) between α-synuclein and DAT immunostainings. Scale bar = 10 µm C, D Quantification of colocalization analysis. MCCs between α-synuclein and vGLUT1, and α-synuclein and DAT were normalized to control treatments and are indicated as mean values (± SEM). Nested t-test (t (6) = 3.3 showed significance (n = 3, for α-synuclein/vGLUT1, n = 3 (t (178) = 6.6) for α-synuclein/DAT, with 30 individual measurements per n). *P < 0.05, ****P < 0.0001