Fig. 2

LRRK2 inhibition reduces relative distance between α-synuclein and vGLUT1 in primary hippocampal neurons A Confocal microscope images of non-expanded and 2.4 expanded primary neurons immunostained for α-synuclein (green), vGLUT1 (red) and Homer1 (blue). Scale bar = 10 µm. B Representative synapses from 7 days treatment with DMSO control and LRRK2 kinase inhibitor treatment (10 nM MLI-2 Scale bar = 5 µm.) C, D Quantification of distance measurements. (Nested t-test t = (116) = 6.5. ****P < 0.0001, n = 2, 25 measurements per sample)