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Table 1 List of identified peptides derived from r-αSyn incubated with CK2 and ATP for 7 days

From: Tyrosine 136 phosphorylation of α-synuclein aggregates in the Lewy body dementia brain: involvement of serine 129 phosphorylation by casein kinase 2

Query Start End Observed Mr(expt) Mr(calc) ppm Score Expect Peptide
WT
5284 44 58 802.9034 1603.792 1603.797  − 2.97 65 3.5E−07 K.TpKEGVVHGVATVAEK.T
11012 46 80 1172.286 3513.836 3513.808 8.02 51 8.4 E−06 K.EGVVHGVATpVAEKTKEQVTN*VGGAVVTGVTAVAQK.T
8325 59 80 746.3851 2236.134 2236.147  − 5.77 47 0.000021 K.TKEQVTpNVGGAVVTGVTAVAQK.T
8336 59 80 1120.068 2238.122 2238.115 3.44 21 0.0083 K.TpKEQ*VTN*VGGAVVTGVTAVAQK.T
11170 59 96 924.9822 3695.9 3695.914  − 3.77 76 2.4 E−08 K.TpKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVK.K
5009 81 96 779.8777 1557.741 1557.744  − 1.95 88 1.6 E−09 K.TVEGAGSpIAAATGFVK.K
12198 98 140 1228.245 4908.951 4908.947 0.96 20 0.011 K.DQLGKN*EEGAPQEGILEDMPVDPDN*EAYEMPSpEEGYQDYEPEA
12214 98 140 1232.002 4923.98 4923.958 4.6 34 0.00044 K.DQLGKN*EEGAPQEGILEDMoPVDPDNEAYEMPSEEGYQDYpEPEA
12225 98 140 1235.754 4938.985 4938.969 3.36 21 0.01 K.DQLGKNEEGAPQEGILEDMoPVDPDNEAYEMoPSEEGYQDYpEPEA
12226 98 140 1235.756 4938.994 4938.969 5.24 30 0.0011 K.DQLGKNEEGAPQEGILEDMoPVDPDNEAYpEMoPSEEGYQDYEPEA
12228 98 140 1236.002 4939.979 4939.953 5.31 23 0.0073 K.DQLGKNEEGAPQEGILEDMoPVDPDN*EAYEMoPSEEGYQDYpEPEA
12229 98 140 1236.003 4939.982 4939.953 5.91 26 0.0032 K.DQ*LGKNEEGAPQEGILEDMoPVDPDNEAYEMoPSEEGYQDYpEPEA
11769 103 140 1092.433 4365.703 4365.693 2.34 21 0.0077 K.NEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYpEPEA
11770 103 140 1456.242 4365.704 4365.693 2.61 18 0.017 K.NEEGAPQEGILEDMPVDPDNEAYEMPSpEEGYQDYEPEA
11805 103 140 1461.57 4381.688 4381.688 0.11 24 0.004 K.NEEGAPQEGILEDMPVDPDNEAYEMoPSpEEGYQDYEPEA
11806 103 140 1461.572 4381.693 4381.688 1.19 22 0.0069 K.NEEGAPQEGILEDMPVDPDNEAYEMoPSpEEGYQDYEPEA
11807 103 140 1461.572 4381.694 4381.688 1.45 28 0.0015 K.NEEGAPQEGILEDMoPVDPDNEAYEMPSpEEGYQDYEPEA
11808 103 140 1096.431 4381.696 4381.688 1.93 25 0.0034 K.NEEGAPQEGILEDMPVDPDNEAYEMoPSpEEGYQDYEPEA
11809 103 140 1461.576 4381.707 4381.688 4.54 28 0.0017 K.NEEGAPQEGILEDMPVDPDNEAYEMoPSpEEGYQDYEPEA
11851 103 140 1100.425 4397.671 4397.682  − 2.69 31 0.00073 K.NEEGAPQEGILEDMoPVDPDNEAYEMoPSpEEGYQDYEPEA
11855 103 140 1466.906 4397.696 4397.682 3.13 31 0.00086 K.NEEGAPQEGILEDMoPVDPDNEAYEMoPSEEGYQDYpEPEA
11856 103 140 1466.908 4397.701 4397.682 4.29 24 0.004 K.NEEGAPQEGILEDMoPVDPDNEAYEMoPSEEGYQDYpEPEA
11870 103 140 1467.57 4399.687 4399.651 8.37 24 0.0039 K.NEEGAPQEGILEDMoPVDPDN*EAYEMoPSpEEGYQDYEPEA
S129A
3243 11 23 691.3577 1380.701 1380.701  − 0.32 28 0.0014 K.AKEGVVAAAEKTpK.Q
1528 22 32 570.2711 1138.528 1138.538  − 9.45 46 0.000028 K.TpKQGVAEAAGK.T
4891 44 58 802.8981 1603.782 1603.797  − 9.67 60 9.1 E−07 K.TpKEGVVHGVATVAEK.T
3201 46 58 688.3341 1374.654 1374.654  − 0.57 42 0.00006 K.EGVVHGVATpVAEK.T
8000 59 80 1119.079 2236.144 2236.147  − 1.15 64 4.2 E−07 K.TpKEQVTNVGGAVVTGVTAVAQK.T
8001 59 80 746.3887 2236.144 2236.147  − 1.02 25 0.003 K.TKEQVTNVGGAVVTGVTpAVAQK.T
5372 81 97 843.9226 1685.831 1685.839  − 4.98 52 6.4 E−06 K.TVEGAGSpIAAATGFVKK.D
  1. p indicates phosphorylation sites; o indicates oxidation site
  2. *Indicates deamidation site