Fig. 8

TBB suppresses αSyn aggregates through reduced Ser129 phosphorylation and increased Tyr136 phosphorylation in SH-SY5Y cells. SH-SY5Y cells treated with TBB (10 and 100 nM) or 0.05% DMSO (0) as vehicle were transfected with (+) or without (−) agitated WT r-αSyn as seed. The lysates from cells were analyzed by SDS-PAGE followed by immunoblotting with a anti-αSyn antibody Syn204, a anti-β-actin antibody, c anti-pS129-αSyn antibody D1R1R, and e anti-pY136-αSyn antibody. Molecular mass markers are indicated in kDa on the left side of each panel. Arrows indicate the top of the gel. Intensity ratios (%) of immunoreactive b αSyn > 25 kDa, d pS129-αSyn, and f pY136-αSyn were quantified in four independent experiments. Data are presented as means ± standard deviation. Statistical significance was determined using one-way ANOVA followed by Tukey–Kramer test. *P < 0.05, **P < 0.01, ***P < 0.001