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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Tyrosine 136 phosphorylation of α-synuclein aggregates in the Lewy body dementia brain: involvement of serine 129 phosphorylation by casein kinase 2

Fig. 6

Blocking Tyr136 phosphorylation promotes phosphorylation of αSyn at Ser129 and Tyr125 induced by CK2. WT r-αSyn or Y136A r-αSyn after 0–40 hours of incubation with CK2 and ATP was analyzed by SDS-PAGE followed by immunoblotting with a anti-pY136-αSyn antibody, b anti-pS129-αSyn antibody ab51253, and d anti-pY125-αSyn antibody. Molecular mass markers are indicated in kDa on the left side of each panel. Arrows indicate the top of the gel. Intensity ratios (%) of immunoreactive c pS129-αSyn, the sum of > 250-kDa and 16-kDa forms, and e > 250-kDa pY125-αSyn after 8 h of incubation were quantified in seven independent experiments. Data are presented as means ± standard deviation. Statistical significance was determined using the 2-tailed Student’s t test. **P < 0.01, ***P < 0.001 versus WT r-αSyn

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