Fig. 4

Ser129 phosphorylation by CK2 elicits Tyr125 and Tyr136 phosphorylation of αSyn. a, b WT r-αSyn or S129A r-αSyn after 0–14 days of incubation with CK2 and ATP was analyzed by SDS-PAGE followed by immunoblotting with a anti-pY125-αSyn antibody and b anti-pY136-αSyn antibody. c, d WT r-αSyn after 0 or 15 days of incubation in the presence (+) or absence (−) of CK2 or ATP was analyzed by BN-PAGE followed by immunoblotting with c anti-pY125-αSyn antibody and d anti-pY136-αSyn antibody. Molecular mass markers are indicated in kDa on the left side of each panel. Arrows indicate the top of the gel. In a–d, one representative blot from three independent experiments is shown