Fig. 6
From: P62 accumulates through neuroanatomical circuits in response to tauopathy propagation
Transsynaptic spread of pathological tau inhibits clearance mechanisms in recipient neurons grown in a microfluidic device. a Timecourse study of the spread of TauRD-YFP inclusions in cortico-cortical network reconstructed in microfluidic devices. Rat cortical neurons in the donor (left) chamber connect via their axons to cortical neuron dendrites in the recipient (right) chamber via synaptic connections in the middle chamber. The three panels show the same cortico-cortical network at DIV8, DIV14 and DIV16. White arrows highlight the progressive aggregation of TauRD-YFP in neurons in the recipient chamber. b Quantification of progressive TauRD-YFP aggregation in neurons in the donor chamber (top panel) and in the recipient chamber (bottom panel). c Confocal immunofluorescence images showing the colocalization between TauRD-YFP aggregates (red), p62 (green) and Map2 (white), in the donor (top panel) and recipient (bottom panel) neuron chambers at DIV 16 in a microfluidic device