Fig. 1

Tau transfer and clearance dysfunction in the seeded PS19 mouse. a DS9 (tau competent seeds) and DS1 (control) injections on each side of the dentate gyrus in the same PS19 mouse. Image source: Allen brain atlas. b–c Immunofluorescence images: MC1 (tau with a pathological conformation—green), p62 (marker of ubiquitinated cargos destined to degradation—white) and DAPI (nuclei—blue) staining on PS19 mice on the DS1 injected side (upper panel) and DS9 (lower panel) for the dentate gyrus. (b) and the entorhinal cortex (c). Scale bar represents 30 μm. d–e DAB staining of the hippocampus, entorhinal and perirhinal cortices of PS19 mice on the DS1 injected side (upper panel) and DS9 (lower panel) for MC1 (d) and p62 (e). Scale bar represents 200 μm. f–g Quantification of the number of positive neurons is shown for MC1 (f) and p62 (g) for the dentate gyrus, CA1, CA3, subiculum and the entorhinal, perirhinal and frontal cortices. Data is normalized to the DS1 side, paired t-test for each region. h Correlation between the number of MC1 and p62 positive neurons in each injected PS19 mouse and for each measured region, on both the DS1 and the DS9 side