Fig. 4

Analysis of the perivascular compartment in AQP4 deficient mice. a and b Assessment by transmission electron microscopy revealed no notable differences in morphology of the endothelium (a1 and b1, false coloured blue), intramural cells (a2 and b2, false coloured green) or basement membrane (a3 and b3, false coloured orange) between wild-type control and AQP4 deficient mice. The red arrows in (a) indicate the location of glial AQP4 in wild-type control mice. When analysed for mean surface area, capillaries in AQP4 deficient mice showed larger lumens and thicker vessel walls (C). The ratio of endothelium/intramural cells or basement membrane were not significantly altered (d). Each box plot represents the range of data from two mice. The scatter plots represent the means of repeated measures (20 per mouse, n = 2)