Table 1 Antibodies used in flow cytometry
From: Chemogenetic modulation of sensory neurons reveals their regulating role in melanoma progression
Antigen | Fluorochrome | Clone | Company |
|---|---|---|---|
CD3 | eFluor450 | 145-2C11 | ThermoFhisher |
CD8a | eFluor 450 | 53–6.7 | ThermoFhisher |
CD11c | eFluor 450 | N418 | ThermoFhisher |
LIVE/DEAD | Acqua | Â | ThermoFhisher |
Streptavidin | Pacific Orange | Â | ThermoFhisher |
CD45 | Super Bright 600 | 30-F11 | ThermoFhisher |
TCR gamma/delta | Super Bright 645 | eBioGL3 | ThermoFhisher |
CD4 | Alexa Fluor 488 | GK1.5 | ThermoFhisher |
F4/80 | FITC | BM8 | Hycult |
NK1.1 | PE-eFluor 610 | PK136 | ThermoFhisher |
CD8a | PerCP-Cyanine5.5 | 53–6.7 | ThermoFhisher |
Ly-6G | PerCP-eFluor 710 | 1A8-Ly6g | ThermoFhisher |
IL-17A | PerCP-Cyanine5.5 | eBio17B7 | ThermoFhisher |
CTLA-4 | PE-Cyanine7 | UC10-4B9 | ThermoFhisher |
FoxP3 | Alexa Fluor 647 | 3G3 | ThermoFhisher |
CD3e | Cyanine5 | 500A2 | ThermoFhisher |
Ki67 | AlexaFluor 700 | SolA15 | ThermoFhisher |
PD-1 | APC-eFluor 780 | J43 | ThermoFhisher |
Ly-6C | APC-eFluor 780 | HK1.4 | ThermoFhisher |
IFN-γ | APC-eFluor 780 | XMG1.2 | ThermoFhisher |
CD11b | Biotin | M1/70 | Biolegend |