Fig. 7

Tumor-draining lymph nodes present an increase in effector CD8 + T-cells after overstimulation of sensory neurons, while the number of lymphocytes in tumor non-draining lymph nodes doesnt change. A Schematic representation of the collected tumor draining lymph nodes. TIL from tumor-draining lymph nodes of B16F10–inoculated Nav1.8-Cre-/hM3Dq + (n = 14) and Nav1.8-Cre + /hM3Dq + (n = 13) mice were analyzed. B Absolute number of CD4 + , CD8 + , γδ, NKT and NK cells from tumor-draining lymph nodes of B16F10–inoculated mice. C IFN-γ and IL-17 were quantified in CD4, CD8, γδ, NKT and NK cells. D Schematic representation of the collected tumor non-draining lymph nodes. TIL from tumor non-draining lymph nodes of B16F10–inoculated Nav1.8-Cre-/hM3Dq + (n = 14) and Nav1.8-Cre + /hM3Dq + (n = 13) mice were analyzed. E Absolute number of CD4 + , CD8 + , γδ, NKT and NK cells from tumor non-draining lymph nodes of B16F10–inoculated mice. F IFN-γ and IL-17 were quantified in CD4, CD8, γδ, NKT and NK cells. Cytokines levels were measured in cells from tumor-draining and tumor non-draining lymph nodes of B16F10–inoculated Nav1.8-Cre-/hM3Dq + and Nav1.8-Cre + /hM3Dq + mice. Data are shown as mean ± SEM. Unpaired t test, *.01 < P < .05; **.001 < P < .01; ****P < 0.0001