Fig. 5

Sensory neurons overstimulation prevent the increase of immune checkpoint markers in tumor infiltrating CD8 + T cells and CD4 + T cells. Immune cells from tumors of B16F10–inoculated mice were analyzed ex vivo in Nav1.8-Cre⁻/hM3Dq⁺ (n = 14) and Nav1.8-Cre⁺/hM3Dq⁺ (n = 13) mice. Column charts show proportion of CTLA-4 (A, D, G, J, M, P), PD-1 (B, E, H, K, N, Q) and CTLA-4/PD-1 co-expressing (C, F, I, L, O, R) CD4 + T cells (A, B, C), CD8 + T cells (D, E, F), γδ T cells (G, H, I), NKT cells (J, K, L), NK cells (M, N, O), and Treg cells (P, Q, R) from tumors of B16F10–inoculated mice. Data are shown as mean ± SEM, Unpaired t test, *.01 < P < .05; **.001 < P < .01