Fig. 4

Sensory neurons overactivation improves anti-tumor immunity by decreasing tumor-infiltrating immunosuppressive cells, increasing dendritic cells and by promoting CD4 + T, CD8 + T, γδT, NKT, and NK-cell infiltration. Immune cells from B16F10–inoculated mice were analyzed ex vivo in Nav1.8-Cre⁻/hM3Dq⁺ (n = 14) and Nav1.8-Cre⁺/hM3Dq⁺ (n = 13) mice. Column charts show the proportion of PMN/MDSC (A) Neutrophils (B) and Dendritic cells (C) quantified in the tumor microenvironment. (D-I) TIL from B16F10–inoculated Nav1.8-Cre-/hM3Dq + (n = 14) and Nav1.8-Cre + /hM3Dq + (n = 13) mice were analyzed ex vivo. Absolute number of CD4 + T cells (D), CD8 + T cells (E), γδ T cells (F), NKT cells (G), NK cells (H), and Treg cells (I) from the melanomas of B16F10–inoculated mice. Data are shown as mean ± SEM, Unpaired t test, *.01 < P < .05; **.001 < P < .01