Fig. 4

RG7388 was able to reach the brainstem and exert therapeutic efficacy in an orthotopic DIPG xenograft model. A The pharmacokinetic profile shows the RG7388 concentration level in plasma and CSF (mean ± SEM, n = 2 per time point). B The pharmacokinetic profile shows the RG7388 concentration level in cerebral hemispheres and brainstem (mean ± SEM, n = 2 per time point). C Log values of bioluminescence signals for each group on day 21 and day 42 respectively (left). Acquired on day 42, representative bioluminescence signal of two mice assigned to treatment and vehicle group (right). P-values based on Student’s t-test. ***P < 0.001. ns = non-significant. D Kaplan–Meier curve of treatment and vehicle group. N = 9 for each group. P-values based on log-rank test. ****P < 0.0001. E The expression of p21 of mouse brain samples from vehicle or RG7388 treatment group on day 42. Scale bar: 100 μm. Mean ± SEM, n = 3 for each group. P-values based on chi-squared test. ***P < 0.001