Fig. 1

RG7388 selectively suppresses proliferation of TP53 wild-type DIPG cells. A Top dependent genes of TP53 wildtype, PPM1D amplification or truncated mutant cell lines. B MDM2 Gene Effect (CERES) across four subgroups. C Ten DIPG cell lines were treated for three days with RG7388 at varying concentrations. Relative viabilities were calculated by normalizing luminescence values for each treatment condition to DMSO treated wells. D The viabilities of the five TP53 wild-type DIPG lines treated with DMSO, 50 nM and 100 nM RG7388 for 2 and 4 days. Relative viabilities were calculated by normalizing luminescence values for each time point to Day 0 at the same treatment conditions. Mean ± SEM, n = 3 independent studies for each condition. P values based on Two-way ANOVA. *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001. ns = non-significant. E Relative Caspase-3/7 activity after treatment with RG7388 for 24 h, normalized to DMSO treatment in TP53 wild-type DIPG cell lines. Mean ± SEM, n = 3 independent studies for each condition. P values based on Student’s t-test. *P < 0.05. **P < 0.01. ****P < 0.0001. ns = non-significant