Fig. 18

Perivascular microglial activation. MHCII expression is associated with microglial activation. Shown are perivascular amoeboid Iba1⁺ microglia (red). Some microglia are also expressing MHCII (green) and extend transcellular processes across the nuclei of Lochkern-like cells. a, Merged images, b Iba1 staining (red); c MHCII staining (green); and d DAPI staining (blue). Insert in d shows an enhanced view of a Lochkern-like cell. Arrows indicate the location of the Lochkern-like cells with hollow nuclei and the transcellular microglial processes (Iba1⁺ and MHCII⁺). Cellular blebs indicate that MHCII⁺ microglia appear to be undergoing apoptosis. Scale bar, 15 µm a–d; 5 μm insert in d