Fig. 6

Ribosome stall-induced aberrant APP.C99 species seed amyloid β-42 aggregation. a Immunoblots showing effects of genetic manipulations of RQC and CAT-tailing related factors on the levels of the various APP.C99 species in fly muscle extracts. >  > : FL-APP.C99; * putative CAT-tailed, internally-stalled APP.C99; > : non-CAT-tailed, internally-stalled APP.C99. b Immunoblots showing the effect of ZNF598 RNAi on the level of stalled APP.C99 in elav > APP; BACE1 fly brain extract. c Fluorescent images showing induction of Aβ42 aggregation by APP.C99 in HEK293 cells stably transfected with Aβ42-YFP, and the modifying effect of RNAi or OE of select RQC and CAT-tailing related factors. Fixed cells were directly imaged. Bar graph shows data quantification. Scale bar, 10 µm. d Immunoblots showing effect of artificial addition of different-lengthed AT tails on the aggregation of Aβ42. e Immunostainings showing effect of artificial addition of different-lengthed AT tails on the aggregation of Aβ42 in HeLa cells. 6E10 detects all Aβ42, whereas mOC78 detects aggregated Aβ42. Scale bar, 5 µm. Error bars, ± SEM; ^∗P < 0.05, ^**P < 0.01. Immunoblots represent at least 3 independent repeats