Fig. 7

Astrocytes in WMI have increased expression of OPN. Staining of anti-OPN (green) and anti-GFAP (red) with DAPI nuclear counterstaining was performed and OPN-expressing astrocytes in the white matter were counted. (A-L) Examples of OPN-GFAP double-labeled cells in control (A–D), WMI (E–H), and GMH (I-L) cases (arrowhead: OPN-GFAP double-labeled cells, star: OPN single-labeled cells). (M) Density of OPN⁺-GFAP⁺ cells. Scale bar = 25 µm