Fig. 6

OPN expression is induced in amoeboid microglia. Double labeling of anti-OPN (green) and anti-Iba1 (red) with DAPI nuclear counterstaining was performed, and double-positive cells were counted in the white matter. (A-L) Representative immunofluorescence images from control (A-D), WMI (E–H), and GMH (I-L) cases (arrowhead: OPN-Iba1 double-labeled cells, star: OPN single-labeled cells). (M) Density of OPN⁺-Iba1⁺ cells. Scale bar = 25 µm