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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: TNF-mediated neuroinflammation is linked to neuronal necroptosis in Alzheimer's disease hippocampus

Fig. 5

TNFR1-mediated necroptosis signaling is activated in the CA1 neurons in AD hippocampus. a Analysis of mRNA levels of the TNFR1, TNFR2, and FADD genes in the hippocampus grey matter in AD cases (n = 30) and controls (n = 14). b, c Representative western blots of the soluble and membrane bound forms of TNF and TNFR1, TNFR2, and FADD (full blots are shown in Additional file 6: Fig. 6) and their quantification in AD (n = 15) and control cases (n = 9), normalized to GAPDH. Data are represented as mean ± SEM, *p < 0.05, ***p < 0.001. d Representative immunofluorescence image of pRIPK3 (red, top panel) and pMLKL (red, bottom panel) and TNFR1 (pink) in NeuN + (green) CA1 neurons with DAPI + cell nuclei (blue) in an AD case (scale bar = 20 µm). e) Representative immunofluorescence image of pRIPK3 (green), pMLKL (red) and TNFR1 (pink) with DAPI + cell nuclei (blue) in an AD case (scale bar = 20 µm)

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