Fig. 2

Necrosome formation in the AD hippocampus. a Representative images of pRIPK3 IHC in the hippocampal CA1 region from a control and AD case (scale bar = 20 µm) and the quantification of pRIPK3 + cell density and percentage of pRIPK3 + neurons in AD (n = 22) and control (n = 10) cases. b Representative images of pMLKL IHC in the hippocampal CA1 region from a control and AD case (scale bar = 20 µm) and the quantification of pMLKL + cell density and percentage of pMLKL + neurons in AD (n = 22) and control (n = 10) cases. Data are represented as mean ± SEM, ****p < 0.0001. c Representative immunofluorescence image of RIPK1 (green) and pMLKL (red) with DAPI + cell nuclei (blue) in CA1 pyramidal neurons of an AD case (scale bar = 20 µm). d Representative immunofluorescence image of pRIPK3 (green) and pMLKL (red) with DAPI + cell nuclei (blue) in CA1 pyramidal neurons of an AD case (scale bar = 20 µm). Some neurons are pRIPK3 + , but pMLKL- (arrowhead). e Correlation analysis between pRIPK3 and pMLKL cell densities in AD cases (r = 0.93, p < 0.0001). f Co-immunoprecipitation analysis of pRIPK3 (Co-IP) with MLKL (IP) in the hippocampal grey matter lysates from 2 AD and 2 control cases. Correlation analysis by Spearman comparison