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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: TNF-mediated neuroinflammation is linked to neuronal necroptosis in Alzheimer's disease hippocampus

Fig. 1

Activation of necroptosis signaling in AD hippocampus. a Analysis of mRNA levels of the RIPK1, RIPK3, and MLKL genes in the hippocampus grey matter in AD cases (n = 30) and controls (n = 14). b Representative western blots of RIPK1, RIPK3/pRIPK3, and MLKL/pMLKL. Full blots are shown in Additional file 6: Fig. 6. c Quantification of RIPK1, RIPK3, MLKL, pRIPK3, and pMLKL protein levels in AD (n = 20) and control cases (n = 10), normalized to GAPDH. d Western blot analysis of MLKL from hippocampal grey matter tissue lysate extracted in native conditions, showing the presence of oligomers. Quantification of the MLKL oligomers (~ 250 kDa) in the AD (n = 14) and control (n = 8) cases. For two group comparisons, the Mann–Whitney test was used. Data are represented as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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