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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: TREM2 modulates differential deposition of modified and non-modified Aβ species in extracellular plaques and intraneuronal deposits

Fig. 4

TREM2T66M causes increased deposition of pSer8-Aβ and N3pE-Aβ in APP/PS1 transgenic mouse brains. a Representative pSer8-Aβ stained female 12 M old-APP/PS1ΔE9-TREM2 and APP/PS1ΔE9-TREM2T66M mouse brain sections (color scale bar = 35 mm, represents min/max pixel intensities, 10x). b Dot plots representing number of plaques/mm2, c Box and whiskers plots showing sizes of plaques (µm2) stained with N3pE-Aβ, nmAβ, and Aβ (2964) antibodies in the SSC, RSC, and DG of female 12 M APP/PS1ΔE9-TREM2T66M and APP/PS1ΔE9-TREM2 mice. d Representative images showing deposition of pSer8-Aβ, nmAβ, and Aβ (2964) in SSC of female 12 M-APP/PS1ΔE9-TREM2T66M  and APP/PS1ΔE9-TREM2 mice (scale bar = 50 µm, 40xW). e Ratio of pSer8/nmAβ in SSC (t(3.953) = 2.863, *p = 0.0464), RSC (t(2.915) = 3.94,*p = 0.0307), and DG (t(3.743) = 3.018,*p = 0.0427) of female 12 M-APP/PS1ΔE9-TREM2T66M compared with APP/PS1ΔE9-TREM2 mice. f Representative N3pE-Aβ stained female 12 M-APP/PS1ΔE9-TREM2 and APP/PS1ΔE9-TREM2T66M mouse brain sections (color scale bar = 35 mm, represents min/max pixel intensities, 10x). g Dot plots representing number of plaques/mm2, h Box and whiskers plots representing size of plaques (µm2) stained with N3pE-Aβ and Aβ (4G8) antibodies in the SSC, RSC, and DG of the female 12 M-APP/PS1ΔE9-TREM2T66M and APP/PS1ΔE9-TREM2 mice. i Representative images showing deposition of N3pE-Aβ, nmAβ, and Aβ (4G8) in SSC of female 12 M-APP/PS1ΔE9-TREM2T66M  and APP/PS1ΔE9-TREM2 mice (scale bar = 50 µm, 40xW). j Ratio of N3pE-Aβ/nmAβ in SSC, RSC, and DG of female 12 M-APP/PS1ΔE9-TREM2T66M compared with APP/PS1ΔE9-TREM2 mice. Each dot represents average value of number of plaques or ratio/animal. The box and whiskers plots represent min/max values of distribution of plaque size with the median (shown by the line dividing the box) and the dot plots represent mean ± SEM (n = 3 animals, color- blue (APP/PS1ΔE9-TREM2) and orange (APP/PS1ΔE9-TREM2T66M), unpaired t-test with Welch's correction for analysis of the number and ratio while Mann–Whitney test for plaque size, nsp > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001 or ****p < 0.0001)

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