Fig. 3

TREM2 deletion leads to increased N3pE-Aβ deposits in 5xFAD transgenic mouse brains. a Representative images showing deposition of N3pE-Aβ, nmAβ, and Aβ (4G8) in SSC of male 15 M old-5xFAD-TREM2^−/− and  TREM2^+/+ mice (scale bar = 200 µm, 20x). b Dot plots representing  number of plaques/mm², c Box and whiskers plots showing plaque size (µm²) stained with N3pE-Aβ and Aβ(4G8) antibodies in the SSC, RSC, and DG of the male 15 M-5xFAD-TREM2^−/− and  TREM2^+/+ mice. d Ratio of N3pE-Aβ/nmAβ in the SSC, RSC, and DG of male 15 M-5xFAD-TREM2^−/− compared with TREM2^+/+ mice. e Representative images showing deposition of N3pE-Aβ, nmAβ, and Aβ (4G8) in SSC of male 5 M-5xFAD-TREM2^−/− and  TREM2^+/+ mice (scale bar = 200 µm, 20x). f Dot plots representing number of plaques/mm², g Box and whiskers plots showing plaque size (µm²) stained with N3pE-Aβ and Aβ (4G8) antibodies in the SSC, RSC, and DG of male 5 M-5xFAD-TREM2^−/− and  TREM2^+/+ mice. h Ratio of N3pE-Aβ/nmAβ in the SSC, RSC, and DG of male 5 M-5xFAD-TREM2^−/− compared with TREM2^+/+ mice. Each dot represents average value of the number of plaques or the ratio of N3pE-Aβ/nmAβ per animal. The box and whiskers plots represent min/max values of distribution of plaque size with the median (shown by the line dividing the box) and the dot plots represent mean ± SEM (n = 5 animals, color- blue (5xFAD-TREM2^+/+) and green (5xFAD-TREM2^+/+), unpaired t-test with Welch's correction for analysis of the number and ratio while Mann–Whitney test for plaque size, ^nsp > 0.05, *p < 0.05, **p < 0.01 or ****p < 0.0001)