Fig. 2

TREM2 deletion leads to increased deposition of pSer8-Aβ in 5 M old 5xFAD transgenic mouse brains. a Dot plots representing number of plaques/mm², b Box and whiskers plots showing  plaque size (µm²) stained with pSer8-Aβ, nmAβ and Aβ (2964) antibodies in the SSC, RSC, and DG of male 5 M old-5xFAD-TREM2^−/− and  TREM2^+/+ mice. c Representative images showing deposition  of pSer8-Aβ, nmAβ, and Aβ (2964) in SSC of male 5 M-5x-FAD-TREM2^−/− and  TREM2^+/+ mice (scale bar = 50 µm, 40xW). d Ratio of pSer8/nmAβ in SSC, RSC and DG in male 5 M-5xFAD-TREM2^−/− compared to TREM2^+/+ mice. Each dot represents average value of the number of plaques or the ratio of pSer8-Aβ/nmAβ per animal. The box and whiskers plots represent min/max values of distribution of plaque size with the median (shown by the line dividing the box) and the dot plots represent mean ± SEM (n = 5 animals, color- blue (5xFAD-TREM2^+/+) and green (5xFAD-TREM2^−/−), unpaired t-test with Welch's correction for analysis of the number and ratio while Mann–Whitney test for plaque size, ^nsp > 0.05, *p < 0.05, **p < 0.01 or ****p < 0.0001)