Fig. 1

TREM2 deletion leads to increased deposition of pSer8-Aβ in 15 M old 5xFAD transgenic mouse brains. a Representative pSer8-Aβ stained male 15 M old-5xFAD-TREM2^+/+ and TREM2^−/− mouse brain sections (color scale bar = 35 mm, represents min/max pixel intensities, 10x). b Dot plots representing number of plaques/mm², c Box and whiskers plots showing plaque size (µm²) stained with pSer8-Aβ, nmAβ and Aβ (2964) antibodies in the SSC, RSC, and DG of male 15 M-5xFAD-TREM2^−/− and  TREM2^+/+ mice. d Representative images showing deposition of pSer8-Aβ, nmAβ, and Aβ (2964) in SSC of male 15 M-5xFAD-TREM2^−/− and  TREM2^+/+ mice (scale bar = 50 µm, 40xW). e Ratios of pSer8/nmAβ in SSC (t(7.41) = 7.856,****p < 0.0001), RSC (t(7.696) = 7.611,****p < 0.0001), and DG (t(7.945) = 6.345,***p = 0.0002) of male 15 M-5xFAD-TREM2^−/− compared with TREM2^+/+ mice. Each dot represents average value of the number of plaques or the ratio of pSer8-Aβ/nmAβ per animal. The box and whiskers plots represent min/max values of distribution of plaque size with the median (shown by the line dividing the box) and the dot plots represent mean ± SEM (n = 5 animals, color- blue (5xFAD-TREM2^+/+) and green (5xFAD-TREM2^−/−), unpaired t-test with Welch's correction for analysis of the number and ratio while Mann–Whitney test for plaque size, ^nsp > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001 or ****p < 0.0001)