Fig. 1
From: Generation of human chronic wasting disease in transgenic mice
Western blotting of PK-resistant PrPSc in PMCA products. The PK-resistant PrP from PMCA products in the presence of non-CJD human brain homogenates containing PrPC seeded with a trace amount of various CWD elk homogenates containing PrPSc was detected by western blotting probed with the monoclonal anti-PrP antibody 3F4. a, The PrPC substrate used in the PMCA reaction was from human brain homogenates of non-CJD cadavers with either PrPC-129MM (MM#1 and MM#2 two individual cases) or PrPC-129VV (VV#1 case). The PrPSc seeds were from brain homogenates of three CWD elk cases (#1, #2 and #3) and a non-CWD elk (#4) was used as a negative control. b, The PrPC substrate used in the PMCA reaction was from human brain homogenate of the non-CJD cadaver with PrPC-129VV (VV#1 case). The PrPSc seeds were from brain homogenates of six CWD elk cases (#5 through #8, #1, and #2) and a CWD deer case (#9). Two sonicators (S1 and S2) were used for PMCA as a comparison and duplication