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Table 3 List of primary and secondary antibodies used for Western immunoblotting

From: Absence of Apolipoprotein E is associated with exacerbation of prion pathology and promotes microglial neurodegenerative phenotype

Antigen Abbreviation Host Dilution Source Catalog # (Clone)
Beta-actin β-actin Mouse 1:10,000 Sigma-Aldrich, St. Louis, MO A2228 (AC-74)
Cluster of differentiation 230 CD230 Mouse 1:5,000 BioLegend, San Diego, CA 808,001 (6D11)
Glial fibrillary acidic protein GFAP Rabbit 1:15,000 Dako/Agilent Technologies Santa Clara, CA Z0334
Human apolipoprotein E ApoE Goat 1:7,000 Meridian Life Science Inc., Memphis, TN K74180B
Murine apolipoprotein E ApoE Mouse 1:5,000 Gift of D.M. Holtzman, Washington University St. Louis, MO [57] (HJ6.3)
Goat IgG, HRP-linked secondary antibody   Donkey 1:30,000 Santa Cruz Biotechnology Inc., Dallas, TX Sc-2020
Rabbit IgG F(ab’)2 specific, HRP-linked secondary antibody   Donkey 1:30,000 GE Healthcare Bio-Science Corp., Pittsburgh, PA NA9340
Mouse IgG F(ab’)2 specific, HRP-linked secondary antibody   Donkey 1:30,000 GE Healthcare Bio-Science Corp., Pittsburgh, PA NA9310