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Table 2 List of secondary antibodies used for immunohistochemistry

From: Absence of Apolipoprotein E is associated with exacerbation of prion pathology and promotes microglial neurodegenerative phenotype

Antigen

Host

Conjugate

Source

Catalog #

Anti-mouse IgG

Goat

Alexa 594

Jackson Immuno Research Labs, West Grove, PA

115–585-146

Anti-mouse IgG

Goat

Alexa 488

Jackson Immuno Research Labs

115–545-166

Anti-rabbit IgG

Goat

Alexa 488

Jackson Immuno Research Labs

111–545-144

Anti-rabbit IgG

Goat

Alexa 594

Jackson Immuno Research Labs

111–585-144

Anti-rabbit IgG

Donkey

Alexa 488

Jackson Immuno Research Labs

711–545-152

Anti-rabbit IgG

Goat

Biotin

Vector Laboratories, Burlingame, CA

BA-1000

Anti-rat IgG

Goat

Alexa 594

Jackson Immuno Research Labs

112–585-143

Anti-sheep IgG*

Donkey

Alexa 594

Invitrogen, Waltham, MA

A-11016

  1. Working dilution 1:500, except* diluted 1:400