Fig. 8
Attenuated microglial phagocytic in prion infected Apoe−/− mice. a and c Representative LSCM images of CD68/Iba1 and TREM2/Iba1 double immunostained microglia in the S1 cortex of 22L inoculated WT and Apoe−/− mice at 23 wpi, respectively. b, d Quantitative analysis of CD68/Iba1 and TREM2/Iba1 ratio in the S1 cortex, respectively; and e enumeration of % TREM2+ microglia in the layer V of the S1 cortex in indicated animal groups (n = 8–12 mice/group in b and n = 6–7 mice/group in d and e). f Analysis of Trem2 mRNA level. The qRT-PCR results are presented as the ΔCT values (n = 3–11 mice/group). Microglia in 22L Apoe−/− mice feature attenuated expression of CD68 and TREM2 protein, which are microglial phagocytic activation markers compared to 22L WT mice, despite upregulation of CD68 and Trem2 transcript. b and f p < 0.0001 (ANOVA); *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (Holm’s-Sidak’s post hoc test). d and e **p < 0.01, and ****p < 0.0001 (two-tailed t-test with Welch’s correction). Values in b, d, e, and f represent mean + SEM. Scale bar: 10 μm in a and c