Fig. 7

Prion infected Apoe^−/− mice feature marked upregulation of MGnD and M0 transcriptomic profiles. Shown are transcript heatmaps of nanoStringTM nCounter® expression data from NBH and 22L inoculated WT and Apoe^−/− mice, which were killed at 23 wpi. Microglia specific transcripts are split into a MGnD–specific, and b M0 (Homeostatic)—specific cassettes. Hierarchical cluster analysis shows salient separation of 22L Apoe^−/− mice from other experimental groups both for MGnD- and M0- specific cassettes, while 22L WT mice separate from NBH inoculated WT and Apoe^−/− mice within the MGnD-specific cassette but not within M0- specific cassette. c Fold change of nanoStringTM nCounter® values for MGnD and selected homeostatic (M0) microglia markers in 22L WT and 22L Apoe^−/− mice (n = 3 mice/group) relative to those in averaged NBH controls (n = 5 mice/group). c p < 0.05 to p < 0.0001 (ANOVA) analyzed for each gene separately; *p < 0.05 **p < 0.01 ***p < 0.001, and ****p < 0.0001 22L Apoe^−/− or 22L WT vs averaged NBH WT and NBH Apoe^−/−; ^♦p < 0.05, ^♦♦p < 0.01, ^♦♦♦p < 0.001, and ^♦♦♦♦p < 0.0001 22L Apoe^−/− vs 22L WT (Holm’s-Sidak’s post hoc test following significant ANOVA). Data in c represent mean + SEM