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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Anatomic survey of seeding in Alzheimer’s disease brains reveals unexpected patterns

Fig. 2

Reproducibility of seeding data. a To determine variation in seeding by region, we randomly selected a subset of 50 samples from the 1st sample set (tested in Exp I and shown in Fig. 1) and gathered a second set of punches from these brain regions (2nd sample set). Exp II tested seeding in the 2nd sample set (50 samples total plus controls). In Experiment III, seeding of both sample sets was tested a second time (100 samples total plus controls). In summary, both 1st and 2nd sample sets were tested in 2 separate runs, with the second run following a freeze–thaw cycle (Run 1 and 2). b and c show the plots for coefficient of variation versus FRET average for samples and controls in experiments II and III. The graphs were generated in the same way as for Experiment I in Fig. 1b and c. All control samples fall below the seeding threshold of 0.35%. Color code: lipofectamine (yellow), buffer (pink), tau negative human brain tissue (blue), tau knockout mouse brain tissue (green), samples from individuals 1–20 (black). d and e show a correlation analysis (linear regression) to test the reproducibility of seeding for the same sample sets between different experimental runs. f and g show the correlation analysis to test the reproducibility between different punches from the same brain region. Note that control samples were not included in graphs d to g

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