Fig. 6
Low-grade peripheral inflammation affects Aβ transport across the blood-CSF barrier in vivo and in vitro. a Quantification of Aβ1-40 and Aβ1-42 in CSF and plasma (n = 5–8). b Expression of the genes P-gp and Lrp2 in CP (n = 5). c Representative images of LRP2 immunostaining of CP. Scale bar: 20 μm. d Quantification of the relative red staining of LRP2 in CP (n = 5 per group). e Schematic diagram of Aβ transcytosis analysis in an CP epithelial transport system using fluorescently labeled Aβ peptides. f movement of scrambled Aβ1-425-FAM (left) and Aβ1-42TAMRA (middle) from the basolateral to the apical chamber, and normalized Aβ transcytosis quotient of Aβ1-42 from the basolateral to the apical chamber (right) (n = 3). g Representative images of LRP2 staining in primary CP epithelial cells. h Quantification of the relative red staining of LRP2 in primary CP epithelial cells (n = 3). Mean ± SEM, two-way ANOVA Bonferroni’s post hoc test for multiple comparisons (a, b, d), one-way ANOVA Bonferroni’s post hoc test for multiple comparisons f Nonparametric Mann–Whitney U test h. *p < 0.05, **p < 0.01, ***p < 0.001