Fig. 9

Reduced TREM2 expression led to reduced mRNA expression of DAM stage 2 markers in AD-tau-injected 5XFAD mice. qPCR analysis of DAM stage 2 genes in cortex and hippocampus samples of AD-tau-injected 5XFAD × TREM2^+/+, 5XFAD × TREM2^+/− and 5XFAD × TREM2^−/− mice. Data from male (n = 3) and female (n = 3, except n = 1 for 5XFAD × TREM2^+/+) mice were pooled, and samples of left and right hemisphere from each mouse were used as individual data points (data by sex and hemisphere can be found in Additional file 1: Fig. S6). TREM2 was below the threshold of detection in the 5XFAD × TREM2^−/− mice and thus expression is shown as zero. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001