Fig. 2

PLX3397 treatment led to a reduction of plaque-associated microglia in AD-tau-injected 5XFAD mice. a Plaque-associated microglia were reduced in the cortex of PLX3397-treated (PLX) compared to control (CTR) AD-tau-injected 5XFAD mice. b Example image of Iba1-positive microglia after thresholding (left), and subsequent overlaying of the thresholded image onto Aβ-stained plaques to identify plaque-associated microglia (right). Only the Iba1 signal that was plaque-associated was quantified, resulting in mean Iba1 area per plaque. Quantification of plaque-associated Iba1 area in the cortex (c) and subiculum (d). Scale bar represents 50 µm