Fig. 5

IDH-mut astrocytoma immune clustering showed five distinct clusters associated with tumor grade and CDKN2A/B deletion. a UMAP clustering of IDH-mutant astrocytoma cohort (N = 734) based on immune cell proportion (Non-cancer part scaled from 0 to 1) showed subtype specific clustering. b Five optimum number of cluster obtained in IDH-mut cohort by using k-means clustering with Nbclust method. c Six major Immune cell type proportions shown by boxplots for each cluster indicated significant difference between immune cells distribution across all cluster. Y axis represents non-cancer cell proportion of particular immune cell scaled from 0 to 1. Each box plots depicted, boxes indicate interquartile range with central bar indicating median and whiskers indicating the range. Blue dot represents mean value of the proportion. T-test and Wilcoxon test (p value shown) were used to calculate statistical significance. d Sample proportions as tumor grade 1, 2 & 3 in each cluster (N = 370; significance based on Fisher's exact test; p value < (0.0001, 0.001, 0.01, 0.05, 1; symbols = "****", "***", "**", "*", "ns”). e Cluster 2 and 5 showed significant difference between sample proportion with CDKN2A/B deletion. Horizontal bars between cluster bars with asterisks represent chi- square test based comparison with significant p value (< 0.001)