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Table 2 Transmission of sporadic CJD isolates (10% brain homogenates) into mice expressing the human PrP (methionine, valine, at codon 129)

From: Prion strains associated with iatrogenic CJD in French and UK human growth hormone recipients

Isolate number

Codon 129

PrPres type

Origin

Brain area

TgMet129

TgVal129

Identified strain(s)

Passage 1

Passage 2

Passage 1

Passage 2

Survival time

PrPres type

Survival time

PrPres type

Survival time

PrPres type

Survival time

PrPres type

23

MM

1

Fr

Frontal cortex

230 ± 13

1

201 ± 11

1

336 ± 8

1

284 ± 5

1

M1CJD

24

MM

1

UK

Frontal cortex

226 ± 4

1

ND

 

326 ± 9

 

ND

 

M1CJD

25

MV

1

Fr

Frontal cortex

276 ± 19

1

205 ± 7

1

296 ± 13

1

282 ± 9

1

M1CJD

26

MV

1

UK

Frontal cortex

197 ± 6

1

209 ± 17

1

289 ± 13

1

289 ± 8

1

M1CJD

27

MV

1

Fr

Frontal cortex

205 ± 16

1

220 ± 13

1

258 ± 33

2

184 ± 6

2

M1CJD + V2CJD

28

MV

2

UK

Frontal cortex

470 ± 23

1

211 ± 12

1

184 ± 9

2

180 ± 6

2

M1CJD + V2CJD

29

MV

2

Fr

Frontal cortex

375 ± 45

1

205 ± 5

1

251 ± 66

2

168 ± 3

2

M1CJD + V2CJD

30

MV

2

UK

Frontal cortex

578 ± 28

1

492 ± 70

1

219 ± 17

2

174 ± 3

2

V2CJD

31

MV

2

Fr

Caudate

518 ± 84

1

571 ± 14

1

180 ± 8

2

161 ± 8

2

V2CJD

32

VV

2

Fr

Frontal cortex

626 ± 85

1

548 ± 24

1

198 ± 7

2

170 ± 7

2

V2CJD

33

VV

2

Fr

Frontal cortex

521 ± 65

1

216 ± 1

1

188 ± 13

2

166 ± 6

2

M1CJD + V2CJD

  1. Transgenic mice that express the Met129 (tgMet), Val129 (tgVal) human PrP were inoculated intra-cerebrally (20µL per mouse) with sporadic Creutzfeldt–Jakob (sCJD) brain tissue homogenates (frontal cortex or caudate nucleus) from patients originating (orig.) from France (Fr), or the United Kingdom (UK). The sCJD patients displayed different PRNP genotypes at codon 129 (MM: homozygous Met129, VV: homozygous Val129, MV: heterozygous Met/Val129) and PrPres Western blot isoforms (type 1 or type 2). After the first passage, brain tissue from clinically affected mice were pooled and used for a second passage in the same line. The PrPres WB isoforms (type 1 or type 2) identified in mouse brains are reported for each two passages. Survival times are shown as mean ± standard deviation (SD). 100% attack rate transmission were observed in all cases. ND: not done. At the exception of isolate 24, transmission data have already been use in a previous publication. The prion strain(s) identified in each isolate (strain typing based on survival time and vacuolar lesion profile in the brain) are indicated in the table. For full data (lesion profile data and WB PrPres typing) please refer to Cassard et al. [9]