Fig. 2
From: Prion strains associated with iatrogenic CJD in French and UK human growth hormone recipients
PrPres western blot profiles in the brains of human PrP-expressing mice (TgHu) inoculated with human growth hormone iatrogenic CJD (hGH-iCJD) cases. Transgenic mice that express the Met129 (tgMet) or Val129 (tgVal) human PrP were inoculated intra-cerebrally (6 mice, 20µL per mouse) with a 10% brain homogenate from hGH- iCJD cases of French and UK origin. Two iterative passages were performed in each line (Table 1). After each passage and in each mouse line the isoform (type 1/type 2) of the PrPres was determined in mice brain by SDS-PAGE and WB with the anti-PrP monoclonal antibody Sha31 (epitope YEDRYYRE). A PrPres type 1 isoform (MM1 sCJD isolate) and type 2 isoform (VV2 sCJD isolate) were included as controls on each gel. The WB results obtain in each line are reported in Table 1