Fig. 3

Effect of extracellular vesicle (EV) secretion by the choroid plexus (CP) after intracerebroventricular (icv) injection of Aβ oligomers (AβO) on mixed cortical cultures (MCC). (a) Overview of the experimental setup. The Figure was partially created with BioRender. (b) Nanoparticle Tracking Analysis (NTA; NanoSight) quantification of the amount of particles in medium of CP explants that were isolated from C57BL/6J mice mice 3 h after icv injection of scrambled peptide (black) or AβO (grey) (n = 5 and n = 10) and cultured for 16 h in Opti-MEM. (c, d) Cytokine and chemokine analysis of CP explant and MCC supernatant (c) or MCC cells (d). CP explants were isolated from C57BL/6J mice mice 3 h after icv injection of scrambled peptide (black) or AβO (grey) (n = 5 and n = 10) and cultured for 16 h in Opti-MEM, after which the supernatant was collected for Bio-Plex analysis. MCC were incubated with the complete secretome of CP explants derived from scrambled peptide (black) or AβO (grey) injected mice or incubated with qEV enriched EVs separated from the secretome of CP explants derived from AβO (white) injected mice. 24 h after incubation the supernatant and cells were collected and analyzed using respectively Bio-Plex assay for KC, MCP1, IL6 and RANTES and qRT-PCR analysis for Kc, Mcp1, Il6 and Rantes (n = 5)