Fig. 6

IHC staining of formalin fixed cerebellar and pontine tissue sections from an MSA patient with antibodies to full-length or carboxy truncated form of αSyn. Antibody 94-3A10 that reacts with amino acid region 130–140 in αSyn and antibodies (10A4, 4H11, 1B1 and 2G5) specific for carboxy truncated forms of αSyn were used to stain tissue from the cerebellum white matter region and pons of an MSA patient. In the pons sections, the pontine nuclei (PN) and transverse fibers (TF) regions are identified. Arrows depicts GCIs. Tissue sections were stained with the αSyn antibodies indicated in the top left corner. All sections were counterstained with hematoxylin. Scale bar = 100 μm