Fig. 2

Western blot analyses to demonstrate the specificities of new monoclonal αSyn antibodies. Immunoblot analyses was performed using 1–102, 1–103, 1–115, 1–119, 1–122, 1–125, 1–129, and full-length (FL; 1–140) recombinant αSyn protein as indicated above each lane and as described in “Material and Methods”. 210 ng of recombinant protein was loaded per lane and membranes were probed with the primary antibodies listed above each panel. Relative motilities of molecular mass markers are indicated on the left side of each blot