Fig. 5
DSS colitis and RGS10 deficiency impact nigrostriatal dopamine pathways and increase susceptibility to MPTP. A Relative mRNA levels encoding tyrosine hydroxylase (TH) in SNpc (n = 5–8 per group (groups distinguished by sex, genotype, and treatment), two-way ANOVA, treatment effect p < 0.001, Tukey’s post hoc), B representative images of TH+ (brown) cells in the RGS10+/+ SNpc (4 × magnification, scale bar = 250 μm), and C TH, D dopamine transporter, and E VMAT2 protein in striatum measured by western blot and normalized to total protein (n = 7–9 per group (groups distinguished by sex, genotype, and treatment); two-way ANOVA, treatment effect p < 0.01 for all except VMAT2 for females, genotype effect p < 0.05 for all, Tukey’s post hoc) from mice given H2O or DSS followed by saline or MPTP. Letter(s) centered above groups reflect results of post hoc tests. Groups that do not share any letter are significantly different from one another